Heat Adaptation Alters Escherichia coli O157:H7 Membrane Lipid Composition and Verotoxin Production
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چکیده
منابع مشابه
Adaptation of Escherichia coli O157:H7 to pH alters membrane lipid composition, verotoxin secretion, and resistance to simulated gastric fluid acid.
The influence of adaptation to pH (from pH 5.0 to 9.0) on membrane lipid composition, verotoxin concentration, and resistance to acidic conditions in simulated gastric fluid (SGF) (pH 1.5, 37 degrees C) was determined for Escherichia coli O157:H7 (HEC, ATCC 43895), an rpoS-deficient mutant of ATCC 43895 (HEC-RM, FRIK 816-3), and nonpathogenic E. coli (NPEC, ATCC 25922). Regardless of the strain...
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Sixty-four verotoxin-producing (VT+) Escherichia coli strains were analyzed for VT1- and VT2-specific DNA sequences and for production of hemolysin. Strains of human origin were of the following serotypes: O157:H7 or H-, O111:H8 or H-, O26:H11, O114:H4, and rough:H7. Strains of serotypes O157:H7, O113:H21, O116:H21, and rough:H- were from cattle, while those of serotype O139:K12:H1 were from pi...
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Microbial synthesis of free fatty acids (FFA) is a promising strategy for converting renewable sugars to advanced biofuels and oleochemicals. Unfortunately, FFA production negatively impacts membrane integrity and cell viability in Escherichia coli, the dominant host in which FFA production has been studied. These negative effects provide a selective pressure against FFA production that could l...
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The content of fatty acids extracted from the membranes of E. coli MC 1061 harboring the wild-type dnaKdnaJ alleles and its delta dnaJ and delta dnaKdnaJ derivatives was compared. It was demonstrated that dodecanoic acid was missing in NPLs fraction extracted from both mutants grown at 42 degrees C. Phospholipids extracted from mutant strains were deprived of hexadecanoic acid methyl ester and ...
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The excimer-forming fluorophore dipyrenylpropane has been used to measure the relative fluidity of total membranes isolated from Escherichia coli grown at 30 or 45 degrees C, or exposed to a heat-shock from 30 to 45 degrees C for various periods of time. Parallel experiments were performed using [35S]methionine pulse-labeling of cells, to study the induction of heat-shock proteins (HSPs) at dif...
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ژورنال
عنوان ژورنال: Applied and Environmental Microbiology
سال: 2003
ISSN: 0099-2240,1098-5336
DOI: 10.1128/aem.69.9.5115-5119.2003